Liberação controlada de fármacos em scaffolds eletrofiados de policaprolactona (PCL) e piperina (PIP) na engenharia tecidual óssea

Abstract

Polymeric drug scaffolds have been intensively studied in the field of orthopedic surgery due to their ability to release the drug in a controlled and targeted manner at the desired location. In this context, electrospinning is a promising technique to produce scaffolds using biodegradable and biocompatible polymers. In the present study, scaffolds Polycaprolactone (PCL) with Piperine (PIP) incorporated at different concentrations (1, 3 and 5%) were obtained by electrospinning. The electrospun groups PCL, PCL/PIP 1%, PCL/PIP 3% and PCL/PIP 5% were analyzed by UV-vis spectroscopy to identify the incorporated drug and evaluate its controlled release. In addition, bone repair was verified by Raman spectroscopy using ᶹ1PO43- vibration data obtained by in vivo analysis. The morphology and diameter of the nanofibers were obtained by scanning electron microscopy (SEM). The scaffolds PCL, PCL/PIP 1%, PCL/PIP 3%, PCL/PIP 5%, showed diameters 0.0015±0.002μm, 1.08±0.007μm, 1.09±0.02μm, 0.59±0.016μm, respectively. The release of piperine was pronounced after 15 days. Therefore, at 30 days, the PCL/PIP 1% scaffold released 64%±0.00062, while the PCL/PIP 3% and PCL/PIP 5% scaffolds released only 16.06±0.00012 and 9.08%±0.00031, respectively. Raman spectroscopy, by statistical analysis, showed the emphasis of significant bone repair in the PCL/PIP1% group at 15 days compared to PCL/PIP 3% and 5%, as predicted in the sustained release of these scaffolds. However, at 30 days, the statistical data showed a pattern of bone repair between the groups.