Perfil fitoquímico, atividade antioxidante e citogenotoxicidade da catingueira (Poincianella bracteosa (Tul.) L.P. Queiroz)

Abstract

Poincianella bracteosa (Tul.) L. P. Queiroz, known as catingueira, is a species widely used to treat kidney infections, hypertension, intestinal colic and anemia. O Assess the phytochemical profile, antioxidant activity and cytogenotoxicity of catingueira (Poincianella bracteosa). Infrared spectroscopy revealed characteristic bands of group O-H in EE and polar fractions (EF and AF), indicating the presence of hydroxylated compounds in Catingueira. Methyl (CH3) and methylene (CH2) groups were observed, suggesting the presence of an aliphatic chain in HF. In the verification of the chromatographic profiles by CLAE-DAD in the EF and AF fractions, it was possible to register the presence of two classes of secondary metabolites, hydrolyzable tannins (ellagitanins and galotanins) and flavonoids. The analysis by ESI-IT / MSn allowed the identification of 7 phenolic compounds: gallic acid, quinic acid, ellagic acid, amentoflavone, corilagine, chebulatic acid and castalagine. EF had a higher content of total phenolics (441.23 ± 1.82 mg EAG / g), while HF registered a higher content of total flavonoids (87.77 ± 4.34 mg EQ / g). Greater antioxidant activity was observed in EF (EC50 25.06 ± 0.1 µg / mL). Cytotoxic effect was observed for all fractions in the A. cepa assay. In the antigenotoxicity test, the protective effect of EF and AF that acted by modulating the mutagenic action of MMS was observed, mainly inhibiting the occurrence of micronuclei. EF was considered to be the most promising, as it was not genotoxic, had protective activity against the damage induced by MMS and also presented cytotoxic activity. This is the first report of identification of phenolic compounds in the species P. bracteosa.